Identification of a third metalloprotease toxin gene in extraintestinal isolates of Bacteroides fragilis.

نویسندگان

  • G T Chung
  • A A Franco
  • S Wu
  • G E Rhie
  • R Cheng
  • H B Oh
  • C L Sears
چکیده

To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.

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منابع مشابه

Prevalence of fragilysin gene in Bacteroides fragilis isolates from blood and other extraintestinal samples.

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عنوان ژورنال:
  • Infection and immunity

دوره 67 9  شماره 

صفحات  -

تاریخ انتشار 1999